SpectraMax ID5 Plate Reader (Molecular Devices) DescriptionThe SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. style. 8. . Standard concentrations ranged from 1x10-11 M to 1x10-6 M. 3. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. The iD5 reader also measures time-resolved fluorometry (TRF) and fluorescence polarization (FP), and can be expanded to measure time-resolved fluorescence energy transfer (TR-FRET), homogeneous time-resolved fluorescence. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Antigen Quantitation Protocol. Instrument pricing. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. 28 nM to 0. Fluorescence Microplate Reader Comparison Chart. We would like to show you a description here but the site won’t allow us. 1. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 003 fmol 0. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. In addition, the iD5 reader measures TRF and FP and. Key features. Heading. Find regional support. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Introduction. • SpectraMax iD3/iD5 Multi-Mode Microplate Readers • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only)SpectraMax iD5 is a hybrid monochromator and filter-based and only filter-based coupled with “Enhanced TRF moduleis compatible. logistic, and EC. Bldg. Dual injectors feature low dead volume (10 μL), overflow protection and ensure equal mixing across the. com. B; Overview. ; The top 3 importers of Spectramax are India with 365. Instrument setup. QuickSync. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Find regional support. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Here, we describe validation of the microplate reader with the NanoBRET ™ PPI Control Pair, consisting of the interacting protein partners p53 and MDM2, on the SpectraMax iD5 reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax iD5 reader performed all analytical measurements required for this screening platform. In addition, the SpectraMax® iD5 can be expanded to include bottom. Wait 20 seconds. BMG Labtech - FLUOSTAR OMEGA. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Lower limits of detection for ATP determined using the ATPlite . A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. B; Overview. 4. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. Molecular Devices. Category 2 and 3 microplate readers use gradient heating as well. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The calculated LOQ values were 0. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. SpectraMax Injector Cartridge with SmartInject™ Technology. To differentiate between the sensitivity of the Biuret and the Folin assay an experiment was proposed, the importance of assays further needs of investigation to see which is more suitable for and for what. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Explore PDF for more details. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. 1. Category 2 and 3 microplate readers use gradient heating as well. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/µL using just a few microliters of sample. #iD5-STD) Methods ATP standard curve. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Consulte todas as informações sobre o produto leitor de microplacas multimodal de absorvância SpectraMax® iD5 da empresa Molecular Devices. 3. Review date: 30 Nov 2020 | SpectraMax® iD5 Multi-Mode Microplate Reader. EM and SpectraMax M2 are not shown here. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any . . , San Jose, CA, USA). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. SpectraMax L: 36. In addition, the SpectraMax iD5 reader can be expanded. Figure 4. SpectraMax iD5. book Page 1 Wednesday, July 7, 2010 2:09 PMWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Compact designs make these instruments. style. 2 only) SpectraMax M-series Microplate readers. 7°C (gradient heating design). All data are in μM and are n ≥ 3 independent experiments. ALP 005-Altitude Lab Hazard Communication Plan. The SpectraMax iD5 User Guide is a comprehensive document that provides detailed instructions on how to set up, operate, and maintain the SpectraMax iD5 Multi-Mode Microplate Reader. SpectraMax iD5: 81. Buy 6590-0147POS - SpectraMax ID5, ScanLater Set. How to create a user profile, set a pin, and pair an NFC tag on the SpectraMax iD5 Hybrid Microplate Readerthe SpectraMax Para digm Multi-Mode Detection Platform. Application Note: Streamline BCA-Based Protein Quantitation on the SpectraMax iD5 Reader. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. 58% for the detection module and 2. In addition, the SpectraMax® iD5 can be expanded to include bottom. If no sound is heard during initialization, contact technical support. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. 5. Molecular Devicesの全商品を見る. SpectraMax iD3/iD5 + AquaMax Bundle. Spectranet customer portal SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. 概要 - マルチプレートリーダーSpectraMax® iD3/iD5. Figure 4. Sciences). BMG Labtech - FLUOSTAR OMEGA. The PAGE electrophoresis experiments were performed using an instrument (Beijing Liuyi). 5059784G 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 16 EnvironmentalControl 16The assay is fully optimized for use with the SpectraMax® iD5 Multi-Mode Microplate Reader plus injector system with SmartInject™ Technology. 8. 8 x 10-15. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. UK RU ES RO FR DE IN IT. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 96/384-well plate formats. Quantiferon-TB Gold in Tube Kit. 28 nM to 0. application or budget. Whether you are looking for an intuitive, easy-to-use reader for your busy,. 1 x 10-15. Investigation of the RNP activation ability of Cir-amplifier in a classic CRISPR/Cas12a. . SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. In addition, the SpectraMax® iD5 can be expanded to include bottom-read luminescence, TR-FRET, HTRF®, BRET, dual. In addition, the SpectraMax® iD5 can be expanded to include bottom. 1. 4. 8 x 10-15. B) The enhancing effect of NECA was offset by A2AR antagonist, but not by antagonists specific. Customers are the lifeblood of Spinco. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 1 x 10-15. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Expanded wavelength coverage. Refer to “Installing Detection Cartridges” in. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. SERVICES. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 1 Cell Culture. 6 μM C12-FAM in a reaction buffer composed of 1 mM HEPES (pH 8) and 1. In addition, the SpectraMax® iD5 can be expanded to include bottom. spr * SpectraMax ABS Plus SpectraTest ABS2 v1. High Quality Auto Parts and Service since 1938. 2 x 10-14. SpectraMax Mini: 17. 5 mP. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 41 nM were calculated using SoftMax Pro Software. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Prepare your i3/i3x instrument for shipment. The SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. We would like to show you a description here but the site won’t allow us. Other technologies such as imaging. 1. Filterless readers provide consistent results with steady temperature regulation. 6 x 10-15. Alexander Lloyd Irwin started the business in Weyburn, Saskatchewan in 1938, then moved Sandy's Auto Parts to Victoria in 1945. 8. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax iD3/iD5 readers use SoftMax ® Pro Data Acquisition & Analysis. Validate the optical performance of your absorbance . With optimized reagents, option to operate the readers. With optimized reagents, option to operate the readers. Molecular Devices SpectraMax iD5 Multi-Mode kuoppalevylukija on täydellinen laitteisto absorbanssi-, fluoresenssi-, luminesenssi-, aikaerotteisiin fluoresenssi- (TRF) ja viritettäviin fluoresenssi polarisaatio (FP) mittauksiin. See SpectraMax® Microplate Reader User Manual Download Page. The absorbance was measured using a Spectra Max iD5 device (Molecular Devices Inc. SpectraMax® iD5 Multi-Mode Microplate Reader See All Models SpectraMax® iD5 Multi-Mode Microplate Reader by Molecular Devices®. LDH Assay. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. About the reader setup recommendations HTRF assays are available in three different donor/acceptor fluorophore combinations, each requiring specific reader settings for optimal results: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Conclusion. Italic and boldface type are used for emphasis. Kysy tuotteesta. 305 reference level saturation. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 7 mm. The SpectraMax iD3 reader detects fluorescence using monochromators only. Fluorescence and fluorescence polarization. 使用SpectraMax iD5酶标仪的四光栅系 统进行波长选择的荧光检测与使用专门用 于Alexa594示踪剂的滤光片对的荧光检 测进行了比较，SpectraMax iD3酶标仪 仅使用四光栅系统检测荧光。 表1 SpectraMax iD3/iD5酶标仪的推荐设置。使用新批次的微孔板和测定体. 1. Introducing the ImageXpress Micro Confo. i3x. We used a Cytotoxicity LDH Assay Kit-WST (Dojindo Molecular Technologies, Inc. 価格、見積もり、お近くの販売店を. For more sample throughput, the system canHow to start a plate read on the SpectraMax iD5 hybrid multi-mode microplate readerWe would like to show you a description here but the site won’t allow us. The SpectraMaxiD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes -Easily set up protocols, run experiments,. Examples: “Press carefully to engage,” “ Do not press down. HTRF IP standard curve. on Molecular Devices microplate readers: SpectraMax® iD5, i3x, Paradigm®, and M5 Multi-Mode Microplate Readers. 100 fmol 1536-well (8 µL) 0. The SpectraMax iD5 intelligently d rives rapid data acquisition the first time, every time. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. Find regional support. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. SpectraMax L: 36. SpectraMax Mini: 17. The SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax® iD3 は、吸光、蛍光、発光測定モードを搭載しています。 SpectraMax® iD5 は、吸光、蛍光（モノクロメーター／フィルター）、発光、時間分解蛍光（TRF）および蛍光偏光（FP）測定モードを標準搭載、さらなる機能拡張により、発光下方測定. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 41 nM were calculated using SoftMax Pro Software. We offer over 1000 different promotional items everything from coffee mugs to t-shirts that can be. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Spectranet customer portal The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Microplate reader assays requiring infrared (IR) or red-shifted. 8 x 10-15. Select the Driver tab and click Update Driver. Also refer to this Multimode Reader Comparison Chart. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Examples: “Press carefully to engage,” “ Do not. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. 1 x 10-15. ALP 006-Altitude Lab Spill Response. 306 plate air cal fail,low light. For wavelengths in the UV range above 220 nm,. When connecting for the first time to either the SpectraMax L reader or StakMax system. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax Mini: 17. 100 fmol 384-well (75 µL) 0. Results obtained with SpectraMax iD5 (red), i3x (green), and M5e (blue) readers were plotted using a 4-parameter . SpectraMax iD5: 81. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Winnersh Triangle. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. It may be named "Pothos". ScanLater Western Blot Detection Cartridge. Built-in near-field communication (NFC) functionality for access to custom protocols and results with a single tap; Enhanced touchscreen workflows for walk-up usabilityUnlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. The measurement conditions should be set up in the SoftMax® Pro software according to the following indications: Setup TRF moduleSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Standard concentrations ranged from 1x10-11 M to 1x10-6 M. 1. SpectraMax Mini: 17. Plate reading technology - SpectraMax ID5 Multi-mode plate reader Five-mode plate reader with automatic filter identification to read between wavelengths 250nm and 1000nm: comes with built-in absorbance, fluorescence, luminescence, fluorescence polarization (FP) and time-resolved fluorescence (TRF) modes. Talk to an expert. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax iD5 Multi-Mode Microplate Re. 3: The cuvette chamber. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. 7°C (gradient heating design). StakMax Handling Stacker Base Unit. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time. The absorbance was measured at a wavelength of 405 nm (SpectraMax iD5 multi-mode microplate reader, Molecular Devices, USA). 1. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. NFC-based personalization and enhanced touchscreen workflows provide walk-up usability The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Results obtained with SpectraMax iD5 (red), i3x (green), and M5e (blue) readers were plotted using a 4-parameter . SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. 4. 0. 2 x 10-14. LoadingThe SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. BMG LABTECH - CLARIOstar Plus . SoftMax Pro GxP Compliance Software. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. #iD5) Methods. The corrected absorbance is within 5% of the value. spr SpectraMax iD5 SpectraTest ABS2 v1. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. The plate was covered and incubated for one hour at room temperature. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. また、 プレートリーダのメーカー15社一覧 や 企業ランキング も掲載しておりますので是非ご覧ください。. SpectraMax iD5 多功能微孔板读板机具 有光吸收检测功能，可以进行蛋白定量 检测。下面，我们介绍一下如何用 SpectraMax iD5 配套 SoftMax®Pro 软件， 用 Pierce BCA 蛋白检测试剂盒和 Pierce Rapid Gold BCA 蛋白检测试剂盒进行检 测。以上试剂盒均包括标准品牛血清白蛋 The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Table 1. The SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax iD5 多功能微孔板读板机具 有光吸收检测功能，可以进行蛋白定量 检测。下面，我们介绍一下如何用 SpectraMax iD5 配套 SoftMax®Pro 软件， 用 Pierce BCA 蛋白检测试剂盒和 Pierce Rapid Gold BCA 蛋白检测试剂盒进行检 测。以上试剂盒均包括标准品牛血. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. How to view results from a plate read on the SpectraMax iD5 Hybrid Microplate Reader使用配备SpectraMax注射器卡盒的SpectraMax iD3或 iD5读板机获得激动剂浓度-反应曲线，每次获得一种激动剂 浓度。从低浓度到高浓度对每条浓度-反应曲线进行分析测 定，以减少残留。用化合物灌注注射器，然后将装有染料的Download PDF. In addition, the SpectraMax iD5 reader can be expanded Explore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. Overview of the SpectraMax® i3x Multi-M. In this application note, Molecular Devices demonstrates how the SpectraMax ® iD5 Multi-Mode Microplate Reader with SoftMax ®. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. 1. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Erlotinib (nM) Inhibition of EGF-induced phosphorylation of ERK1/2 (T202/Y204) by . Description 1. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 8. 2 x 10-14. The SpectraMax® iD3 and iD5 measure absorbance, fluorescence, and luminescence. SpectraMax iD5 reader, which performed FP measurement on the Valita Titer assay plate. 8. 1. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. SpectraMax iD5 は、 タグにより、 スライドとフィルターの構成を認識するための フィルター識別コードを自動的に検知します。 複雑な作業は不要で、フィルター追加・交換の ワークフローが簡略化されています。Filterless readers provide consistent results with steady temperature regulation. It may be installed in the top or bottom read detection cartridge transport. #iD5-STD) Methods ATP standard curve. Recommended settings for SpectraMax iD5 and i3x readers. If no sound is heard during initialization, contact technical support. Table 1. Expanding The Boundaries of Microplate Reader Functionality - The. DNA standards ranging from 50 pg/mL to 1 μg/mL were detected using the Quant-iT PicoGreen dsDNA Assay Kit and SpectraMax readers (data from the SpectraMax iD5 reader are shown, but other SpectraMax readers gave similar results). SpectraMax M5e: 118. 3. This five-mode microplate reader with automatic filter identification and western blot capability is the complete laboratory. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Our absorbance plate readers feature our PathCheck Sensor. QuickSync. 4. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. It is important to determine the excitation and emission wavelengths that provide the best sensitivity and dynamic range for the. Expanding on the feature set of the SpectraMax iD3, which was released in early 2017, the new product is. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Example: [Drawer]. 8. Figure 1. We are a Canadian custom apparel and promotional company here in Victoria, BC. The Kdvalues were 0. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. 50. Whether you are looking for an intuitive, easy-to-use reader for your busy,. SpectraMax ABS SpectraTest ABS2 v1. SpectraMax ABS SpectraTest ABS2 v1. The absorbance of 8-OHdG and nitrotyrosine was measured spectrophotometrically on Spectra Max iD5 (Molecular Devices Inc. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. Sunnyvale, California -- October 5, 2017. The p53 pathway activator nutlin-3 was used to disrupt the p53-MDM2 interaction in a concentration-dependent manner, and the results were analyzed and graphed. 5. Standards were plotted using a log-log curve fit in SoftMax Pro Software. The SoftMax® Touch Software is installed in. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as a 10-fold serial dilution series . Figure 1. Engineered for high performance, the auto dual injector cartridge includes fail safe features. Prepare your i3/i3x instrument for shipment. In addition, the SpectraMax iD5 Qualify the fluorescence performance of the SpectraMax . 1. In addition, the SpectraMax® iD5 can be expanded to include bottom. SpectraMax® iD3 SpectraMax® iD5 SpectraMax® i3x ABSFL LUMI CELL IMAGING WESTERN BLOT INJECTOR TRF FP ALPHA-SCREEN FRET ABS. In addition, the iD5 reader can measure TRF and FP, and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter gene detection with syringe and Western blot detection. 1 x 10-15. In this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. SpectraMax L: 36. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Consistent results are obtained over time offering workflow flexibility. Amongst these. 312 gain calibration failed. The absorbance was measured using a Spectra Max iD5 device (Molecular Devices Inc. Afterwards, cells were treated with a 2-fold dilution series of menadione starting at. spr SpectraMax iD5 SpectraTest ABS2 v1. A brief overview of the SpectraMax iD5 Hybrid Microplate Reader. 96 mL Au@PLL-AEDP-FITC. SoftMax Pro GxP Compliance Software. Tristar 5 Multimode Microplate Reader. In addition, the SpectraMaxThe SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader,. The SpectraMax iD3/iD5 readers use SoftMax ® Pro Data Acquisition & Analysis. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers can measure absorbance, fluorescence and luminescence. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. A High-Throughput, Automated Screening Platform for IgG Quantification During Drug Discovery and Development Introduction The global biologics market was estimated worth over $450 billion in 2022, with an annual growth rate of 10 % to 20301. ALP 004-Altitude Lab Exposure Control Plan. SpectraMax M5e: 118. In addition, the SpectraMax iD5吸収度マイクロプレートリーダー SpectraMax® Mini. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Spectranet customer portalA brief overview of the SpectraMax iD5 Hybrid Microplate Reader. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. With optimized reagents, option to operate the. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalStreamline BCA-based protein quantitation on the SpectraMax iD5 reader. Using the SpectraMax iD3 and iD5 readers with the SpectraMax Injector Cartridge with SmartInject Technology, and FLIPR Calcium 6 and 6-QF Assay Kits, we have demonstrated that it is possible to. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. Unlock your i3/i3x instrument after shipment. The multimode microplate reader SpectraMax iD5 comes with all the relevant optical-sensing measuring technologies built in and can be expanded to include. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Figure 4. Calcium. The following accessories are required: Set of 2. SpectraMax® iD5. No accessories are needed and SoftMax® Pro Software. 1.